Thermo Fisher, V652020, pcDNA™5/FRT/TO Vector Kit

pcDNA™5/FRT/TO Vector Kit pcDNA™FRT⁄TO vector is a 5.
1 kb inducible expression vector designed for use with the Flp-In™ T-REx™ System. The control plasmid, pcDNA™5⁄FRT⁄TO⁄CAT, is also included for use as a positive control for transfection and expression in your Flp-In™ T-REx™ host cell line of choice.
The pcDNA™5⁄FRT⁄TO vector contains the following elements:
• A hybrid human cytomegalovirus (CMV)⁄TetO2 promoter for high-level, tetracycline-regulated expression of the gene of interest in a wide range of mammalian cells
• Multiple cloning site with 10 unique restriction sites to facilitate cloning the gene of interest
• FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ T-REx™ host cell line
• Hygromycin resistance gene for selection of stable cell linesConstitutive or Inducible System: Inducible
Delivery Type: Transfection
For Use With (Application): Regulated Expression
Inducing Agent: Tetracycline
Product Type: Flp-In/T-REx System Expression Vector
Quantity: 20 μg
Selection Agent (Eukaryotic): Hygromycin
Vector: pcDNA
Cloning Method: Restriction Enzyme/MCS
Product Line: Flp-In™, T-REx™, pcDNA™
Promoter: CMV/TO
Protein Tag: Untagged
Unit Size: 20 µg