Waters, 186002017, Atlantis Silica HILIC Column, 100Å, 3 µm, 3 mm X 50 mm, 1/pk

Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches.

Specifications
Chemistry: HILIC
Separation Mode: Hydrophilic Interaction (HILIC)
Particle Substrate: Silica
pH Range Min: 1 pH
pH Range Max: 5 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: No
Silanol Activity: High
Particle Shape: Spherical
Particle Size: 3 µm
Endfitting Type: Waters
Pore Size: 100 Å
Format: Column
Surface Area: 330
System: HPLC
Particle Technology: Silica
USP Classification: L3
Inner Diameter: 3 mm
Length: 50 mm
UNSPSC: 41115709
Brand: Atlantis
Product Type: Columns
Units per Package: 1 pk

Additional Information
Atlantis Silica HILIC Column, 100Å, 3 µm, 3 mm X 50 mm, 1/pk Retain analytes that are simply too polar for reverse-phase chromatography to work by using the Atlantis Silica HILIC Column in conjunction with high organic mobile phases (>80% acetonitrile). When compared to conventional chromatographic reversed-phase methods, HILIC (Hydrophilic Interaction Chromatography) separation mechanisms offer orthogonal analyte selectivity. Peptides, metabolites, and actives are among the substances that the Atlantis Silica HILIC Column is capable of separating. The retention of highly polar molecules is one benefit of HILIC, which also improves LC/ESI-MS response and has direct compatibility with SPE solvents. The lab apparatus additionally provides complementary selectivity when compared to reversed-phase HPLC. You can preserve highly polar basic analytes with volatile mobile phases that are appropriate for compound ionization by ESI-MS using the Atlantis Silica HILIC Column. In drug metabolism investigations, where metabolites are frequently more polar than their parent chemicals and are present in much lower concentrations, this translates into data that display increased sensitivity and lower limits of detection. The Atlantis Silica HILIC Column, in contrast to conventional reversed-phase HPLC columns, not only provides better LC/ESI-MS performance but also direct compatibility with SPE solvents and complimentary selectivity. Additionally, the use of Atlantis Silica HILIC Column delivers superior peak morphologies for polar bases while leveraging quicker and easier method development, in contrast to the standard reversed-phase HPLC columns where severe peak tailing for polar bases is observed. Due to their hydrophilic nature and net positive charge at acidic pH, highly basic polar analytes are exceedingly challenging to retain and separate using reversed-phase chromatography. The Atlantis Silica HILIC Column is essential lab equipment that can be utilized in metabolism, drug discovery, and combinatorial chemistry because it uses HILIC to tackle these difficult separation challenges. You may also want to invest in the LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk , in order to store your analytes safely. Each Waters LCMS Certified vial is made using strictly regulated manufacturing and handling techniques. They are examined using mass spectrometers, and a certificate of analysis displaying the reference and vial scan for the production batch is supplied with them.

FAQ
Does The Atlantis Silica HILIC Column Have Any pH Limitations? The Atlantis Silica HILIC Column listed here can work comfortably within the 1-5 pH range. What Is The Maximum Pressure Recommended For The Atlantis Silica HILIC Column? The Atlantis Silica HILIC Column listed here is able to withstand a maximum of 6,000 psi (415 Bar).