BD, 550295, BD Pharmingen™ Purified Mouse Anti-Rat CD3

Alternative Name: CD3 Complex; T3
Reactivity: Rat (QC Testing)
Isotype: Mouse BALB/c IgG3, κ
Immunogen: PHA-stimulated rat lymph node and spleen cells
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Cytotoxicity, Immunoprecipitation, Inhibition, Stimulation (Reported)
Concentration: 31.25 µg/ml
RRID: AB_393590
Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, goat serum, and ≤ 0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: Purified Mouse Anti-Rat CD3 (Cat. No. 550295) is recommended to test for immunohistochemical staining of acetone-fixed frozen sections and paraffin sections. Tissue tested was rat spleen. The antibody stains T lymphocytes. The isotype control recommended for use with this antibody is Purified Mouse IgG3, κ Isotype Control (Cat. No. 550341). For optimal indirect immunohistochemical staining, the G4.18 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with Purified Mouse IgG3, κ Isotype Control (Cat. No. 553401) as the secondary antibody and Streptavidin HRP (Cat. No. 550946) together with the DAB Substrate Kit (Cat. No. 550880).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).