Merck Protein Sample Prep: Complete Workflow Solutions

When preparing your protein samples, you need a workflow you can rely on. Merck’s Protein Sample Prep Collection provides a comprehensive range of products designed to streamline every step of the process, from cell lysis to protein concentration and buffer exchange. Whether you are working in academic research, pharmaceutical development, or high-throughput screening, Merck’s solutions help ensure that your results are both accurate and reproducible.

Across modern laboratories, protein sample preparation is essential for downstream applications such as mass spectrometry, Western blotting, ELISA, enzyme activity assays, and protein-protein interaction studies. What sets Merck apart is a commitment to quality: our tools are engineered for high recovery rates, low sample loss, and compatibility with a broad spectrum of sample types, ensuring you achieve consistent results every time. With easy-to-use formats and robust product performance, Merck enables you to focus more on discovery and less on troubleshooting.

Protein Extraction & Cell Lysis

The first step in any protein analysis is efficient extraction from cells or tissues. Merck offers a range of protein extraction kits and cell lysis buffers tailored for different sample sources—including bacterial, mammalian, yeast, and plant samples. With optimized reagents such as the ProteoExtract® line, you can disrupt cell membranes effectively while preserving the integrity of your target proteins for downstream analysis.

Iright's Merck extraction products support both gentle and rapid lysis protocols, providing flexibility depending on your workflow and sensitivity requirements. They are compatible with subsequent purification steps and are ideal for applications that demand high protein yield and minimal degradation. By integrating these tools into your process, you lay a solid foundation for downstream purification and analysis.

Popular SKUs:

• ProteoExtract® Complete Mammalian Extraction Kit (539779)
• ProteoExtract® Cytosolic Proteome Extraction Kit (539790)
• ProteoExtract® Subcellular Proteome Extraction Kit (539792)

Protein Purification: Magnetic Beads & FLAG Tag

Following extraction, isolating your protein of interest is critical for accurate analysis. Merck’s purification solutions include magnetic beads, affinity resins, and columns designed for efficient and specific protein isolation. The PureProteome™ Magnetic Beads platform offers rapid, high-capacity purification with minimal hands-on time, suitable for both tagged and native proteins.

For researchers using affinity tags, the Anti-FLAG® M2 Affinity Gel (A2220) provides exceptional selectivity and binding efficiency for FLAG-tagged proteins. These purification tools deliver high-purity proteins compatible with sensitive downstream applications, and their batch-to-batch consistency makes them ideal for both small-scale discovery and large-scale production.

Popular SKUs:

• PureProteome™ Protein A Magnetic Beads (LSKMAGA02)
• Anti-FLAG® M2 Affinity Gel (A2220)
• His•Bind® Resin (654120)
• GST•Bind™ Resin (70541)

Protein Concentration, Desalting & Buffer Exchange

To maximize your assay performance, it is essential to concentrate proteins and remove unwanted salts or contaminants. Merck’s Amicon® Ultra centrifugal filters are the gold standard for rapid, gentle protein concentration and buffer exchange. These devices allow you to process volumes from 0.5 mL to 15 mL with high recovery rates, ensuring your sample is ready for sensitive analytical techniques.

Ultrafiltration and desalting are particularly important before mass spectrometry, crystallography, or activity assays. The Amicon® Pro system enables integrated purification, desalting, and concentration in a single device, simplifying complex workflows. By efficiently removing small molecules and exchanging buffers, you protect protein structure and functionality for reliable downstream results.

Popular SKUs:

• Amicon® Ultra-0.5 mL Centrifugal Filters (UFC5003)
• Amicon® Ultra-15 mL Centrifugal Filters (UFC9030)
• Amicon® Pro Purification System (ACS500)
• Zeba™ Spin Desalting Columns (89889)

Featured Protein Sample Prep Products

Discover the best-sellers trusted by leading laboratories worldwide. These products are recognized for their reliability, efficiency, and compatibility with a wide range of workflows:

• ProteoExtract® Complete Mammalian Extraction Kit (539779): Simplifies mammalian protein extraction with high yields and minimal degradation.
• Amicon® Ultra-15 mL Centrifugal Filters (UFC9030): Industry standard for protein concentration and buffer exchange; delivers fast, consistent results.
• PureProteome™ Protein A Magnetic Beads (LSKMAGA02): Enables rapid immunoprecipitation and high-purity protein recovery, compatible with automated workflows.
• Anti-FLAG® M2 Affinity Gel (A2220): High specificity for FLAG-tagged proteins, ensuring excellent purity for sensitive downstream analysis.
• His•Bind® Resin (654120): Ideal for high-efficiency purification of His-tagged proteins from various lysates.

Each product is backed by detailed protocols and technical support, giving you confidence at every step.

Related Solutions for Upstream/Downstream Applications

Maximize your results by integrating Merck’s Protein Sample Prep Collection with complementary solutions for the entire protein workflow. Pair your extraction and purification tools with Merck’s protease and phosphatase inhibitor cocktails to preserve protein integrity during lysis and storage. For accurate quantification, consider our BCA Protein Assay Kits and Bradford Assay Kits, optimized for compatibility with the sample prep products above.

To further streamline your research, explore sample preservation reagents, dialysis devices, and complete western blotting systems—each designed to work seamlessly within the Merck workflow. By connecting these solutions, you ensure sample quality and analytical accuracy from start to finish.