BD, 550550, BD Pharmingen™ Biotin Mouse Anti-Mouse H-2K[b]

Reactivity: Mouse (QC Testing)
Isotype: Mouse BALB/c IgG2a, κ
Immunogen: Mouse C57BL/6 Splenocytes
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 125 µg/ml
RRID: AB_393744
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures The AF6-88.5 clone reactive against mouse H-2Kb is recommended to test for immunohistorical staining of acetone-fixed frozen sections. Tissues tested were mouse (C57B1/6) spleen and thymus. The antibody stains the H-2Kb molecules found on the surface of all nucleated cells in strains of mice expressing this haplotype. For optimal immunohistochemical staining, the biotinylated antibody should be titrated (1:10 to 1:50 dilution) and developed with Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). The clone AF6-88.5 is not recommended for formalin-fixed paraffin embedded sections.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. An isotype control should be used at the same concentration as the antibody of interest. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.