Agilent, 103576-100, Seahorse XF RPMI medium, pH 7.4, 500 mL.

Seahorse XF RPMI medium, pH 7.4, 500 mL. This is a RPMI-based medium and does not contain phenol red, bicarbonate, glucose, pyruvate, or glutamine. It contains 1 mM HEPES, and the pH of this medium is pre-adjusted to 7.4. To ensure a proper final pH in assay media, Seahorse XF supplements must be used at recommended concentration range. Check Seahorse XF media and buffer selection guide for more details. This medium is validated for use with the Seahorse Real-Time ATP rate assay and the glycolytic rate assay. It is also recommended to use for all Seahorse XF assays.

Specifications:
For Use With: Seahorse XF24 AnalyzerSeahorse XFe24 AnalyzerSeahorse XFe96 AnalyzerSeahorse XFp Analyzer
Shipping Condition: Ambient Temperature
Storage Temp Conditions: Refrigeration required, 2-8°C
Volume: 500 mL

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Seahorse XF RPMI medium is a ready-to-use, bicarbonate-free assay medium formulated at physiological pH 7.4, supplied in a 500 mL bottle. Specifically designed for use with Agilent Seahorse XF Analyzers, this medium supports optimal metabolic flux analysis in live cells. Its composition allows researchers to measure real-time cellular bioenergetics, including mitochondrial respiration and glycolysis, without CO2 buffering interference.

This assay medium is ideal for bioenergetic profiling of mammalian cells, immune cells, and stem cells, providing a controlled environment to analyze mitochondrial function, energy metabolism, and the impact of pharmacological agents. Its transparent formulation is compatible with live cell assays, minimizing background signal and ensuring reproducible data across multiple experiments.

Common applications include studies of cell health, metabolic switching, drug response, and metabolic reprogramming in cancer, immunology, and neuroscience research. The medium is intended for use alongside Agilent Seahorse XF Cell Mito Stress Test, Glycolysis Stress Test, and other XF Cell Metabolic Assays, ensuring robust and consistent results. For optimal performance, the Seahorse XF RPMI medium should be equilibrated to ambient conditions prior to use, and essential supplements such as glucose, glutamine, or pyruvate may be added according to experimental requirements.