Agilent, 103681-100, Seahorse XF RPMI assay medium pack, pH 7.4

Seahorse XF RPMI assay medium pack, pH 7.4. Includes one each of the following products: XF RPMI medium pH 7.4, 500mL (103576-100), XF 1.0 M Glucose Solution 50 mL (103577-100), XF 100 mM Pyruvate Solution 50 mL (103578-100), and XF 200 mM Glutamine Solution 50 mL (103579-100).

Specifications:
For Use With: Seahorse XF24 AnalyzerSeahorse XFe24 AnalyzerSeahorse XFe96 AnalyzerSeahorse XFp Analyzer
Kit Contents: XF 1.0 M Glucose Solution 50 mL (103577-100)XF 100 mM Pyruvate Solution 50 mL (103578-100)XF 200 mM Glutamine Solution 50 mL (103579-100)XF RPMI medium pH 7.4, 500mL (103576-100)

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The Seahorse XF RPMI assay medium pack, pH 7.4, offers a specialized solution formulated for use in real-time cell metabolism measurements. This ready-to-use medium is optimized for compatibility with Agilent Seahorse XF Analyzers, enabling accurate assessment of cellular metabolic activity, including mitochondrial respiration and glycolysis, in diverse cell types.

Featuring a physiological pH of 7.4, the medium supports cell viability while minimizing background interference during the measurement of oxygen consumption rate and extracellular acidification rate. The formulation is serum-free and bicarbonate-free, further reducing variability and enhancing experimental reproducibility.

This pack is ideal for researchers investigating bioenergetic profiles, metabolic switching, drug effects, or nutrient dependencies in adherent or suspension mammalian cells. Use of this medium streamlines assay setup in studies involving cancer metabolism, immunometabolism, and cellular stress responses.

The solution pairs seamlessly with Agilent Seahorse XF24, XF96, XFe24, XFe96, and XFHS Mini Analyzers, and is recommended for integration with Seahorse XF sensor cartridges, calibrant solution, and fluxpaks for optimal performance. With quality manufacturing and stringent control, this medium enhances assay precision and reliability in metabolic research workflows.