Agilent, 5190-2453, Agilent Bio WCX, NP10, 4.6 x 250 mm

Agilent Bio WCX, NP10, 4.6 x 250 mm. A weak cation exchange HPLC column with nonporous particles. Particle size is 10 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio WCX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Charge Variant AnalysisPeptidesProteins
Particle Size: 10 µm
Particle Type: Non-porous
Phase: WCX
Pressure Rating: 275 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio WCX, NP10, 4.6 x 250 mm column is designed for high-performance analysis and purification of biomolecules using weak cation exchange chromatography. Featuring advanced silica-based WCX technology with a 10 nm pore size, this column provides excellent resolution and reproducibility for proteins, peptides, and other charged analytes. Its robust 4.6 x 250 mm dimensions offer high sample capacity and extended separation efficiency, making it ideal for laboratories engaged in biopharmaceutical characterization, quality control, and detailed protein profiling.

This product is optimal for multiple research applications, including charge variant analysis of monoclonal antibodies, detection of protein isoforms, and detailed studies of post-translational modifications. It is compatible with a range of Agilent HPLC and UHPLC instruments, such as the 1100, 1200, and 1260 Infinity Series, as well as most systems from other major manufacturers when standard LC fittings are used. The column integrates seamlessly with Agilent Autosamplers and detectors, enhancing its versatility. For expanded workflow solutions, it can also be used in conjunction with Agilent's complete line of HPLC columns and protein analysis reagents, supporting comprehensive protein research and quality assessment needs.