Agilent, 5190-2460, Agilent Bio WCX, NP10, 2.1 x 50 mm, PEEK

Agilent Bio WCX, NP10, 2.1 x 50 mm, PEEK. A weak cation exchange HPLC column with nonporous particles. Particle size is 10 μm. PEEK hardware enables this column to couple with bio-compatible or bio-inert LC systems.

Specifications:
Brand: Agilent Bio WCX
Column Material: PEEK
Guard Column: No
Hardware: PEEK
Inner Diameter (ID): 2.1 mm
LC Platform: BioInert
Length: 50 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Charge Variant AnalysisPeptidesProteins
Particle Size: 10 µm
Particle Type: Non-porous
Phase: WCX
Pressure Rating: 275 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio Weak Cation Exchange (WCX) NP10 column, measuring 2.1 x 50 mm, is engineered with a robust PEEK housing to ensure chemical resistance and outstanding durability for demanding analytical tasks. Designed expressly for the separation and analysis of biomolecules such as monoclonal antibodies, proteins, and peptides, this WCX column employs a proprietary non-porous particle technology to deliver exceptional resolution, reproducibility, and speed.

Optimized for biopharmaceutical applications, the column provides reliable performance in the analysis of charge variants and isoforms in protein characterization, quality control, and process development workflows. Its compatibility with high-throughput liquid chromatography systems makes it an optimal choice for both research and regulated laboratory environments.

The PEEK construction of the hardware ensures operation with corrosive buffers and high-salt mobile phases commonly required in ion-exchange chromatography, without risk of metal contamination. The column integrates seamlessly with a range of Agilent LC and UHPLC systems, including the 1260 Infinity II and 1290 Infinity II platforms, and is suitable for workflows involving MS detection. For expanded analytical flexibility, matching WCX, SCX, and Bio IEX columns in various dimensions and chemistries are available, allowing tailored solutions to specific analytical challenges in biotherapeutic analysis.