Agilent, 5190-2468, Agilent Bio SAX, NP5, 4.6 x 50 mm, PEEK

Agilent Bio SAX, NP5, 4.6 x 50 mm, PEEK. A strong anion exchange HPLC column with nonporous particles. Particle size is 5 μm. PEEK hardware enables this column to couple with bio-compatible or bio-inert LC systems.

Specifications:
Brand: Agilent Bio SAX
Column Material: PEEK
Guard Column: No
Hardware: PEEK
Inner Diameter (ID): 4.6 mm
LC Platform: BioInert
Length: 50 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: SAX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio SAX NP5 column, measuring 4.6 x 50 mm and constructed with durable PEEK hardware, is designed for advanced anion exchange chromatography of biomolecules such as proteins, peptides, and oligonucleotides. This column features a non-porous 5 micron particle size, enabling sharp peak resolution and efficient separation of charged macromolecules under mild, bio-friendly conditions. Its robust PEEK construction withstands high pH and salt concentrations, making it compatible with a wide range of buffer systems required in biopharmaceutical research, protein characterization, and quality control workflows.

Ideal for laboratories seeking reproducible, high-throughput separations, this column integrates seamlessly with Agilent’s suite of UHPLC and HPLC systems, including 1260 Infinity II and 1290 Infinity II series, as well as equivalent industry-standard platforms. Common usage scenarios include monoclonal antibody charge variant analysis, purity assessment of therapeutic proteins, and detailed mapping of protein isoforms. The column may be paired with Agilent’s BioHPLC detectors, valves, and compatible mobile phases for optimal performance. Researchers can rely on its chemical stability and consistent performance across multiple sample types, ensuring reliable, high-quality data for critical biotechnological and pharmaceutical research applications.