Agilent, 5190-2475, Agilent Bio SAX, NP10, 4.6 x 250 mm, PEEK

Agilent Bio SAX, NP10, 4.6 x 250 mm, PEEK. A strong anion exchange HPLC column with nonporous particles. Particle size is 10 μm. PEEK hardware enables this column to couple with bio-compatible or bio-inert LC systems.

Specifications:
Brand: Agilent Bio SAX
Column Material: PEEK
Hardware: PEEK
Inner Diameter (ID): 4.6 mm
LC Platform: BioInert
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 10 µm
Particle Type: Non-porous
Phase: SAX
Pressure Rating: 275 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio SAX NP10 column, measuring 4.6 x 250 mm and constructed with a durable PEEK housing, is designed for robust and high-efficiency strong anion exchange (SAX) chromatography. Featuring a nonporous 10-micron particle size, this column enables the rapid, high-resolution separation of proteins, nucleic acids, and other biomolecules based on their charge properties. Its chemically inert PEEK material ensures compatibility with a wide range of biological buffers and high-salt gradient elution, reducing metal ion leaching and sample adsorption. Ideal for biopharmaceutical characterization, purity assessment, and quality control, this column is especially suited for laboratories conducting monoclonal antibody analysis, protein fractionation, and complex biomolecule profiling. The column integrates seamlessly with Agilent HPLC and UHPLC systems, including the 1100 and 1260 Infinity II series, and is compatible with many third-party LC instruments. It is also supported by a variety of Agilent mobile phases and buffer solutions for optimized performance. Whether in method development or routine workflows, this SAX column delivers consistent results, excellent lot-to-lot reproducibility, and dependable lifetime, supporting advanced research and reliable analytical throughput.