Agilent, 5190-2485, Agilent Bio WAX, NP5, 4.6 x 250 mm

Agilent Bio WAX, NP5, 4.6 x 250 mm. A weak anion exchange HPLC column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio WAX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: WAX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio WAX, NP5, 4.6 x 250 mm column is engineered for high-resolution anion exchange chromatography, offering robust separation of proteins, peptides, and other charged biomolecules. Featuring a non-porous particle design and high-purity polymeric resin, this column ensures exceptional peak sharpness, reduced carryover, and rapid analyte elution. It is particularly suitable for analyzing monoclonal antibodies, recombinant proteins, charge isoforms, and biotherapeutics, supporting research and development in pharmaceutical, biotechnology, and academic laboratories.

With a 4.6 mm internal diameter and 250 mm length, this column is optimized for use with analytical HPLC and UHPLC systems, including Agilent 1100, 1200, and 1260 Infinity Series, as well as compatible third-party chromatography platforms. The Bio WAX chemistry delivers reliable performance in both salt gradient and pH gradient ion exchange methods, accommodating a range of sample types and loading conditions. The column supports high-throughput workflows through consistent batch-to-batch reproducibility, enabling accurate and repeatable results across complex sample sets. For complete workflow solutions, pair with Agilent sample preparation kits and relevant detection technologies to streamline characterization and quality control processes.