Agilent, 5190-2494, Agilent Bio WAX, NP10, 4 x 10 mm, guard

Agilent Bio WAX, NP10, 4 x 10 mm, guard. A weak anion exchange HPLC guard column with nonporous particles. Particle size is 10 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio WAX
Column Material: Stainless Steel
Guard Column: Yes
Hardware: SS
Inner Diameter (ID): 4 mm
LC Platform: Stainless Steel
Length: 10 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 10 µm
Particle Type: Non-porous
Phase: WAX
Pressure Rating: 275 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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This guard column is specifically engineered for biomolecule separations involving weak anion exchange chromatography. The stationary phase features nonporous 10 micron particles optimized for high-resolution analysis of proteins, peptides, and other large biomolecules. Measuring 4 by 10 millimeters, it is designed to protect and extend the life of corresponding analytical columns by trapping contaminants and particulates from biological samples. It is particularly valuable during the processing of complex matrices encountered in biopharmaceutical research, drug development, biomarker validation, and routine quality control workflows. This guard column integrates seamlessly with a wide range of Agilent liquid chromatography systems and is ideally paired with Bio WAX analytical columns for maximum performance and longevity. Its robust design ensures minimal backpressure and consistent flow, maintaining analytical precision and reproducibility throughout repeated use. The column accommodates diverse mobile phase conditions and is suitable for gradients involving aqueous and organic solvents. Researchers can rely on this guard column to preserve the integrity of their main columns, reduce maintenance, and uphold high data quality across a variety of protein and peptide applications. It is compatible with standard LC instrumentation equipped for biochromatography workflows and enhances overall laboratory productivity.