Agilent, 5190-2522, Agilent Bio SEC-5, 150Å, 7.8 x 150 mm, 5 µm

Agilent Bio SEC-5, 150Å, 7.8 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 0.5-150 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 500 Da
Molecular Weight Upper Limit: 150000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 150 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5, 150 Angstrom, 7.8 x 150 mm, 5 micron column is designed for high-resolution size exclusion chromatography of proteins, peptides, and other biomolecules. With a 150 Angstrom pore size and 5 micron particle diameter, this column offers superb separation efficiency by minimizing secondary interactions, ensuring accurate molecular weight determination and analysis of macromolecular aggregates. Optimized for reproducibility and robustness, it supports demanding applications in protein purity assessment, aggregate analysis, and antibody characterization in pharmaceutical, biotechnology, and academic research environments.

This column is compatible with a wide range of aqueous mobile phases, making it suitable for sensitive biomolecules. Typical usage scenarios include protein therapeutics analysis, quality control testing, and sizing of enzymes, nucleic acids, and other complex samples. The 7.8 mm inner diameter increases load capacity, supporting both analytical and semipreparative workflows.

Agilent Bio SEC-5 columns integrate seamlessly with Agilent HPLC and UHPLC systems, such as the 1260 Infinity II and 1290 Infinity II series, and are also broadly compatible with other major chromatography platforms. Pairing with Agilent's wide range of SEC standards and buffers further streamlines method development. This column delivers consistent performance, durability, and reliability for routine and advanced size exclusion chromatography applications.