Agilent, 5190-2527, Agilent Bio SEC-5, 300Å, 7.8 x 150 mm, 5 µm

Agilent Bio SEC-5, 300Å, 7.8 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 5-1,250 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 5000 Da
Molecular Weight Upper Limit: 1250000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 300 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5, 300 Angstrom, 7.8 x 150 mm, 5 micron column is designed for high-resolution size exclusion chromatography (SEC) of proteins, peptides, and other biomolecules. Engineered with a uniform pore size of 300 Angstroms and a particle size of 5 microns, this column offers optimal separation of proteins ranging from 5,000 to 1,250,000 Da. Its precise silica-based matrix ensures robust performance with excellent reproducibility and minimal sample interaction, making it ideal for analytical and QC applications in biopharmaceutical research, protein characterization, and aggregate analysis.

This SEC column is suitable for use with both HPLC and UHPLC systems and is fully compatible with Agilent 1260 Infinity II Bio LC, 1290 Infinity II Bio LC, and other leading liquid chromatography platforms. It can be integrated into workflows involving protein purity assessment, molecular weight determination, and monitoring of monoclonal antibody aggregates. The column works effectively with common mobile phases including phosphate or Tris buffers, enhancing method versatility for various research or quality control labs. For extended capabilities, it can be used alongside Agilent SEC standards and guard columns. This makes it a trusted solution for accurate and reliable biomolecule size exclusion analysis.