Agilent, 5190-2534, Agilent Bio SEC-5, 500Å, 4.6 x 150 mm, 5 µm

Agilent Bio SEC-5, 500Å, 4.6 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of large biological samples such as adeno associated virus. Resolving range 15 kDa - 5 MDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 15000 Da
Molecular Weight Upper Limit: 5000000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 500 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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This high-performance size exclusion chromatography (SEC) column is designed for precise molecular size analysis of biomolecules such as proteins, peptides, and nucleic acids. With a 500 angstrom pore size, a 4.6 mm inner diameter by 150 mm length, and 5 micron particle size, the column ensures excellent resolution and reproducibility in analyzing molecules ranging broadly in molecular weight. The proprietary silica manufacturing provides high chemical stability, minimizing nonspecific interactions and enabling sharp, Gaussian peak shapes for reliable quantitation.

Ideal for academic, biopharmaceutical, and industrial research laboratories, this column supports applications including characterization of monoclonal antibodies, protein aggregation studies, and quality control of biotherapeutics. It integrates seamlessly with Agilent’s HPLC and UHPLC systems such as the 1260 Infinity II and 1290 Infinity II, and is fully compatible with other leading HPLC instruments. The column’s robust construction ensures long lifespan and consistent performance, even under demanding analytical conditions.

Additionally, it pairs effectively with Agilent SEC standards and mobile phases, supporting straightforward method development and validation. This column is a trusted choice for researchers requiring reproducible and accurate size exclusion separations in both routine and advanced analytical workflows.