Agilent, 5190-6870, Agilent Bio SEC-5, 150Å, 21.2 x 50 mm, 5 µm

Agilent Bio SEC-5, 150Å, 21.2 x 50 mm, 5 µm. A preparative scale size exclusion column for the purification of biological samples. Resolving range 0.5-150 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 50 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 500 Da
Molecular Weight Upper Limit: 150000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 150 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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The Agilent Bio SEC-5 column with 150 angstrom pore size and dimensions of 21.2 x 50 millimeters, featuring 5 micron particle size, is specifically engineered for high-resolution size exclusion chromatography of biological macromolecules. This column is ideal for rapid analysis and preparative purification of proteins, peptides, monoclonal antibodies, and other biomolecules in pharmaceutical and biotechnological research. Its silica-based matrix ensures robust mechanical strength, consistent performance, and minimal non-specific interactions, providing accurate molecular size determinations and aggregate analysis.

Designed for compatibility with Agilent 1260 Infinity II, 1290 Infinity II, and other leading HPLC or UHPLC systems, this column facilitates seamless integration into existing laboratory workflows. The 150 angstrom pore size is optimally suited for separating molecules in the approximate molecular weight range of 5 kDa to 350 kDa, addressing requirements for both analytical and preparative workflows. Common applications include quality control of biotherapeutics, monitoring protein aggregation, and characterization of complex mixtures in biomedical research. The column supports reproducibility in critical quality attribute analyses, making it an essential tool for regulated environments. The robust design also ensures long service life and dependable performance with a wide range of aqueous mobile phases.