Agilent, 877974-910, ZORBAX GF-450, 21.2 x 250 mm, 5 µm, cartridge (PrepHT)

ZORBAX GF-450, 21.2 x 250 mm, 5 µm, cartridge (PrepHT). Use with hardware kit 820400-901

Specifications:
Brand: ZORBAX
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 250 mm
Maximum Temperature: 60 °C
Molecular Weight Lower Limit: 10000 Da
Molecular Weight Upper Limit: 900000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 6 µm
Particle Type: Fully Porous
Phase: GF-450
Pore Size: 300 Å
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 3-8.5

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
The ZORBAX GF-450 21.2 x 250 mm 5 µm cartridge is a high-performance preparative size exclusion chromatography column engineered for biomolecule purification and molecular weight analysis. Optimized for large-scale separation, this PrepHT cartridge effectively resolves proteins, peptides, and other biological macromolecules based on hydrodynamic size. Its 5 µm particle size ensures excellent resolution and throughput, making it ideal for preparative and semi-preparative applications in biopharmaceutical development, protein characterization, and quality control laboratories.

This cartridge is compatible with Agilent PrepHT systems and other preparative liquid chromatography setups using standard 21.2 mm format columns. It is commonly deployed for preparative protein fractionation, aggregate analysis, and desalting steps in both research and process development environments. The robust silica-based material ensures consistent performance with reliable mechanical stability and low nonspecific adsorption.

Researchers benefit from reproducible separations, scalability from analytical to preparative workflows, and compatibility with a wide range of biological buffers. Pairing with Agilent’s portfolio of chromatography instruments, such as the 1260 Infinity II Preparative LC system and matching guard columns, further enhances method flexibility. This column is an excellent solution for laboratories requiring high-purity protein isolations and precise molecular weight investigations.