Agilent, GKE-5020B, AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL

AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL. A recombinant form of PNGase F (400 mU, EDTA free), releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. The same formulation provided in GlykoPrep, ≥ 10 U/mL and EDTA-free. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution

Specifications:
Concentration: 10 U/mL
Enzyme Applications: To obtain efficient deglycosylation of glycoprotein substrates under non-denaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase PLUS and ULTRA (EDTA-Free) are supplied at = 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored.
Enzyme Formulation: 20 mM Tris-HCl, 50 mM NaCl (pH 7.5, EDTA-free formulation).
Enzyme Source: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity: ≥10 units/mg
Enzyme Specificity: Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight: ~35,000 Daltons
Unit: 400 mU
Volume: 40 µL
pH Optimum: 8.6
pH Range: 7.5-9.5

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AdvanceBio N-Glycanase-ultra is a high-purity recombinant Peptide:N-Glycosidase F designed for efficient release of N-linked glycans from glycoproteins. Supplied at a potency of at least 10 units per milliliter and formulated without EDTA, this enzyme is optimized for sensitive glycan analysis protocols. Its EDTA-free composition ensures compatibility with native metals and downstream applications that require metal-dependent enzymes or labeling reagents. It enables rapid and complete deglycosylation under native or denaturing conditions, supporting workflows in biopharmaceutical characterization, glycoprotein profiling, and quality control of biologics.

AdvanceBio N-Glycanase-ultra is suitable for use in sample preparation for HPLC, UHPLC, and LC-MS analysis, facilitating high-throughput and quantitative assessment of N-glycan heterogeneity. The enzyme is ideal for laboratories conducting monoclonal antibody glycan mapping, biosimilar comparability studies, or academic research in protein glycosylation. It integrates seamlessly with AdvanceBio Glycan Mapping columns, Agilent InfinityLab LC and 1290 Infinity II LC systems, and Agilent MassHunter software, ensuring robust and reproducible results. With its fast, complete deglycosylation capabilities and broad compatibility, this reagent is a key component for streamlined and precise glycan analysis in advanced research and regulated environments.