Agilent, GKK-404, 2-AB Labeling Kit

2-AB Labeling Kit. Convenient fluorescent labeling of glycans with 2-AB (2-aminobenzamide) by reductive amination. Any purified glycan or glycan pool with a free reducing sugar may be labeled. Up to 36 samples may be processed (2 batches of 18). Cleanup columns and cartridges must be purchased separately.

For an updated version of this kit please see GKK-804, 2-AB-plus Labeling Kit, in which the 2-AB and Reductant are supplied already in solution. GKK-804 can be used as a direct replacement for GKK-404: when mixed, the GKK-804 2-AB labeling solution will be the same as what is made when all components of GKK-404 are combined. Specifications: Count: 36 Equipment & Reagents Provided by User: Acetic acid (glacial), HPLC gradeAcetonitrile, HPLC gradeCentrifugal evaporator (e.g. Savant, Heto or similar)GlycoClean S CartridgesHeating block, oven or similar dry heater set at 65°CReaction vials (e.g. polypropylene microcentrifuge vials)Water, HPLC grade Kit Configuration: Labeling Kit Label: 2-AB Technique: LC & LC/MS **********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.********** The 2-AB Labeling Kit offers an efficient solution for researchers seeking sensitive labeling of N-linked glycans prior to chromatographic or mass spectrometric analysis. Designed for streamlined workflow, the kit utilizes 2-aminobenzamide (2-AB) chemistry, enabling rapid and reliable fluorescent tagging of released glycans from glycoproteins. This provides enhanced detection sensitivity and facilitates precise quantitation in glycan profiling studies. The protocol is suitable for applications in biopharmaceutical characterization, quality control, and glycomics research, where robust analysis of glycosylation patterns is critical. The kit contains all necessary reagents for efficient labeling, purification, and preparation of samples for downstream separation and detection. It is optimized for compatibility with Agilent’s range of HPLC, UHPLC, and LC-MS instruments, including glycan-specific columns and workflows such as AdvanceBio Glycan Mapping. The product supports parallel processing of multiple samples, accommodating high-throughput environments and routine analysis. Furthermore, compatibility with widely used sample preparation methods and related products ensures seamless integration into existing laboratory protocols. This kit streamlines the process of fluorescent glycan labeling, ensuring consistent and reproducible results across complex biological samples, and supports cutting-edge research in glycoprotein analysis and biotherapeutic development.