Agilent, GKK-500, Chemical Deglycosylation Kit

Chemical Deglycosylation Kit. Employs anhydrous trifluoromethanesulfonic acid (TFMS) to cleave N- and O-linked oligosaccharides from the peptide portion of glycoproteins without significant cleavage of peptide bonds (destroys sugars, leaves protein intact). Up to 24 samples may be processed (2 batches of 12).

Specifications:
Application: Leaves the primary structure of the protein intact C Polypeptide recovery typically 90% C Polypeptide cleavage 80% (both O- and N-linked glycans on glycoproteins, proteoglycans and mucins) C Up to 24 individual samples (2 batches of 12)
Count: 24
Equipment & Reagents Provided by User: Desiccator with suitable drying agentDry ice/ethanol bathGlass syringe gas-tight with Teflon plunger, fitted with a stainless steel needleLyophilizer
Prep time (approx): 5-6 hours

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
The Chemical Deglycosylation Kit from Agilent provides a streamlined solution for efficient removal of N-linked and O-linked glycans from glycoproteins in complex biological samples. This comprehensive kit includes all necessary reagents for chemical deglycosylation, enabling researchers to prepare glycoproteins for downstream analysis in proteomics, biopharmaceutical development, or structural biology studies. Its optimized protocol ensures rapid and consistent deglycosylation while maintaining sample integrity, making it ideal for workflow integration in laboratories studying post-translational modifications, biomarker discovery, or therapeutic antibody characterization. The kit is designed for compatibility with a variety of analytical platforms, including mass spectrometry, HPLC, and capillary electrophoresis systems, supporting both qualitative and quantitative glycan analysis. Paired with Agilent's sample preparation tools, chromatographic systems, and glycan analysis products, this kit enhances data reliability and throughput. Suitable for both manual and automated applications, it accommodates a range of sample types including serum, cell lysates, and purified proteins. This solution enables accurate characterization of glycosylation heterogeneity, facilitating advancements in clinical research and biologic drug development. Comprehensive documentation and technical support ensure smooth implementation in any research environment.