Agilent, GX96-202IAB, AdvanceBio Gly-X deglycosylation and InstantAB labeling module set, 96-ct

AdvanceBio Gly-X deglycosylation and InstantAB labeling module set, 96-ct. Utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid instant labeling of released N-glycans with InstantAB dye. With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation. After a simple cleanup step, the InstantAB-labeled glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods. Includes GX96-100 Gly-X deglycosylation module, GX96-501 Gly-X InstantAB labeling module.

Specifications:
Application: Flexible, high-throughput format:process 1 to 96 samplesInstant labeling with InstantAB:5-minute N-Glycanase (PNGase F) digestion provides unbiased N-glycan release
Count: 96
Equipment & Reagents Provided by User: 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note:Two GlykoPrep heaters, GS150, can be fitted with VWR 13259-260 modular heating blocks.Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note:If you have a vacuum manifold other than the Millipore model suggested, contact us for setup instructions
Kit Configuration: Sample Prep Module Set
Label: InstantAB
Prep time (approx): ~1 hour
Protein Load Range: 1-40 µg
Stability: Samples may be stored at -20°C for at least 6 months or 4°C for up to 5 days
Technique: LC

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This module set provides a comprehensive solution for efficient N-glycan sample preparation, combining rapid enzymatic deglycosylation and high-sensitivity labeling for in-depth glycan analysis. Each kit supports up to 96 samples, enabling high-throughput workflows ideal for biopharmaceutical labs, glycomics research, and quality control environments. The streamlined process facilitates complete deglycosylation and fluorescent labeling using InstantAB technology, delivering samples ready for fluorescent detection in less than three hours.

Optimized for excellent reproducibility and sensitivity, the kit is suitable for use with monoclonal antibodies, recombinant proteins, and other glycoprotein therapeutics. Labeled glycans are compatible with Agilent HPLC, UHPLC, and capillary electrophoresis systems, including the Agilent 1200 Infinity II and 1290 Infinity II LC systems. Processed samples can be directly analyzed using Agilent AdvanceBio Glycan Mapping columns and are suitable for integration with Agilent MassHunter or OpenLab software for data analysis.

Compatible with the full range of AdvanceBio sample preparation and analysis products, this module set streamlines glycan profiling while minimizing hands-on time. Its robust workflow supports regulatory compliance and lot-to-lot consistency, making it an essential tool for detailed glycan characterization in drug development and quality assessment.