Agilent, MX-200-4, MitoXpress Xtra Reagents

MitoXpress Xtra Reagents. Four vials/pk, 1 vial per 96 well plate, and HS Mineral oil, 15 mL, with dropper bottles 4/pk. Contains reagents for four 96 well plates.

Specifications:
Assay Wells: 4 x 96 well plates
For Use With: Other Plate Readers
Shipping Condition: Ambient Temperature
Storage Temp Conditions: Refrigeration required, 2-8°C

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Designed for quantitative assessment of mitochondrial and cellular respiration, this reagent kit enables real-time, high-throughput measurement of oxygen consumption in live cells. The phosphorescent probe provided is sensitive to extracellular oxygen levels, offering a robust and non-invasive method to monitor oxygen consumption rates. Suitable for plate-based fluorescence readers, it is ideal for 96-well and 384-well formats, supporting high-throughput screening in drug discovery, metabolic studies, and bioenergetics research.

Optimized for compatibility with standard fluorescence plate readers, the reagents integrate seamlessly with Agilent’s Seahorse XF Analyzers and are also suitable for use with other major plate reader platforms. The assay supports applications ranging from investigating mitochondrial dysfunction to screening compounds for metabolic impact, and studying cellular responses to hypoxia or mitochondrial toxins. Easy to use and adaptable, this solution streamlines experiments requiring kinetic, time-resolved measurements of oxygen consumption without necessitating cell lysis or extraction steps. For comprehensive metabolic analysis, these reagents can be used alongside products such as Agilent’s Cell Mito Stress Test Kits and ATP Assay Kits. Comprehensive user documentation ensures reliable setup and data interpretation for both experienced and new users in life science research settings.