Agilent, PL1180-5350, AdvanceBio SEC 130Å, 7.8 x 300 mm, 2.7 µm, LC column

AdvanceBio SEC 130Å, 7.8 x 300 mm, 2.7 µm, LC column. A size exclusion column for efficient aggregate analysis of peptides and small proteins. The resolving range is 0.1-120 kDa.

Specifications:
Brand: AdvanceBio SEC
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 100 Da
Molecular Weight Upper Limit: 120000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 2.7 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 130 Å
Pressure Rating: 400 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: 0.02% Sodium Azide
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The AdvanceBio SEC 130Å, 7.8 x 300 mm, 2.7 µm LC column is engineered for robust and accurate size exclusion chromatography of biomolecules such as monoclonal antibodies, biosimilars, and proteins. Featuring precisely controlled, 2.7-micron superficially porous particles and a pore size of 130 angstrom, this column delivers high resolution, excellent reproducibility, and fast analysis times for molecular size determination and aggregate analysis. The column offers exceptional performance for native protein characterization, facilitating high-throughput screening and routine quality control in biopharmaceutical research and development.

Optimized for use with aqueous mobile phases, this column supports analyses in both denaturing and non-denaturing conditions. It is compatible with a wide range of liquid chromatography systems, including Agilent InfinityLab LC series and most standard HPLC and UHPLC instruments. Typical applications include quantification of protein aggregates, molecular weight determination, and the analysis of antibody-drug conjugate heterogeneity. For added flexibility, it integrates seamlessly with Agilent’s AdvanceBio SEC calibration standards and maintenance kits, supporting method development and system suitability procedures. This product provides researchers and analysts with a reliable solution for routine and advanced biomolecular size exclusion workflows in both research and regulated laboratory environments.