Agilent, PL1580-5250, AdvanceBio SEC 120 Å column, 1.9 µm, 4.6 x 300 mm

AdvanceBio SEC 120 Å column, 1.9 µm, 4.6 x 300 mm. A size exclusion column designed to improve resolution and provide faster separation of protein and peptide aggregates and fragments by UHPLC. Resolving range is 1-80 kDa.

Specifications:
Brand: AdvanceBio SEC
Guard Column: No
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 1000 Da
Molecular Weight Upper Limit: 80000 Da
Particle Size: 1.9 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 120 Å
Pressure Rating: 620 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer/0.02% Sodium azide
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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The Agilent AdvanceBio SEC 120 Å, 1.9 µm, 4.6 x 300 mm column is designed for high-resolution size exclusion chromatography applications, particularly suited to biopharmaceutical characterization. Engineered with 1.9 µm particles and a 120 angstrom pore size, this column efficiently separates proteins, peptides, and biomolecules based on molecular size, offering sharp peak resolution and improved accuracy in molecular weight determination. Its robust silica-based stationary phase ensures reliable performance and minimized non-specific interactions, which is crucial for reproducibility in quantitative and qualitative protein analysis.

Typical usage scenarios include aggregate profile assessment, antibody fragment analysis, and the study of protein conjugates in research and quality control environments. The column enables rapid analysis times while maintaining high sensitivity, making it well-suited for high-throughput laboratories.

Compatible with a wide range of inert HPLC and UHPLC systems, including Agilent’s 1100, 1200, 1260, 1290 Infinity, and comparable instruments from other manufacturers, it integrates seamlessly into existing analytical workflows. For broader separation needs, additional pore sizes and column dimensions are available within the AdvanceBio SEC portfolio, supporting method scalability and consistency across development and manufacturing pipelines.