BD, 550278, BD Pharmingen™ Purified Rat Anti-Mouse CD4

Alternative Name: Cd4; CD4 antigen; L3T4; Ly-4; T-cell surface antigen T4/Leu-3
Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Immunogen: A.TH mouse CTL clone A15.1.17
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Blocking, Depletion, Immunoprecipitation (Reported), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 62.5 µg/ml
RRID: AB_393574
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunocytochemistry: The H129.19 antibody is recommended to test for immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin sections. Tissues tested were mouse spleen and thymus. The antibody stains the membranes of thymocytes and a subpopulation of mature T lymphocytes that are MHC class II restricted. The isotype control recommended for use with this antibody is purified rat IgG2a (Cat. No. 559073). For optimal indirect immunohistochemical staining, the H129.19 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with biotinylated polyclonal anti-rat Igs (multiple adsorbed) (Cat. No. 559286) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). More conveniently, the anti-rat Ig HRP detection kit (Cat. No. 551013) that contains the biotinylated secondary antibody, antibody diluent, streptavidin-HRP and DAB substrate can be used for staining. A detailed protocol of the immunohistochemical procedure is available on our website at www.bdbiosciences.com/support/resources . The clone H129.19 is not recommended for formalin fixed paraffin embedded sections.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. An isotype control should be used at the same concentration as the antibody of interest. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.