BD, 550287, BD Pharmingen™ Purified Hamster Anti-Mouse CD54

Alternative Name: ICAM-1; Icam1; Intercellular adhesion molecule 1; Ly-47; MALA-2; MyD10
Reactivity: Mouse (QC Testing)
Isotype: Armenian Hamster IgG1, κ
Immunogen: Not reported
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 125 µg/ml
RRID: AB_393582
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The 3E2 antibody is recommended to test for immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin sections. Tissues tested were mouse spleen and thymus. The clone 3E2 is not recommended for formalin-fixed paraffin embedded sections. The antibody stains lymphocytes, dendritic cells, and macrophages. The isotype control recommended for use with this antibody is purified hamster IgG (Cat. No. 550344). For optimal indirect immunohistochemical staining, the 3E2 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with biotinylated anti-hamster cocktail (Cat. No. 550335) as the secondary antibody and Streptravidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). More conveniently, the Anti-Hamster Ig HRP detection kit (Cat. No. 551012) that contains the biotinylated secondary antibody, antibody diluent, streptavidin-HRP and DAB substrate can be used for staining. A detailed protocol of the immunohistochemical procedure can be found on our website at http://www.bdbiosciences.com/support/resources
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.