BD, 550299, BD Pharmingen™ Purified Mouse Anti-Rat CD11b/c

Alternative Name: Itgam/Integrin, alpha M, C3bi receptor, CR3; Itgad/Integrin, alpha D
Reactivity: Rat (QC Testing)
Isotype: Mouse BALB/c IgG2a, κ
Immunogen: Resident peritoneal cells from (PVG.RT1[c] x PVG.RT1[u]) and (PVG.RT1[c] x PVG.RT1[a]) F1-hybrid rat
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 62.5 µg/ml
RRID: AB_393594
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: This antibody may be used for immunohistochemical staining of acetone-fixed, frozen and zinc-fixed, paraffin sections. This antibody is not recommended for formalin-fixed, paraffin-embedded sections. This antibody has been reported to stain granulocytes and macrophages in tissues such as rat spleen and thymus. The isotype control suggested for use with this antibody is Purified Mouse IgG2a κ Isotype Control (Cat. No. 550339). For optimal indirect immunohistochemical staining, this antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with Biotin Rat Anti-Mouse IgG2a  (Cat. No. 550332) as the secondary antibody and Streptavidin HRP (Cat. No. 550946) together with a DAB Substrate Kit (Cat. No. 550880).