BD, 550530, BD Pharmingen™ Purified Hamster Anti-Mouse CD29

Alternative Name: Itgb1; Integrin beta-1; Fnrb; gpIIa; VLA-4 subunit beta
Reactivity: Mouse (QC Testing), Rat (Tested in Development)
Isotype: Armenian Hamster IgG2, λ1
Immunogen: Purified mouse VLA-4
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 15.625 µg/ml
RRID: AB_393728
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The HM β1-1 antibody specific for mouse CD29 is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse spleen and thymus. The antibody stains lymphocytes and endothelial cells. The isotype control recommended for use with this antibody is purified hamster IgG (550345). For optimal indirect immunohistochemical staining, the HM β1-1 antibody should be titrated (1-10 to 1-50 dilution) and visualized via a three-step staining procedure in combination with anti-hamster IgG cocktail (Cat. No. 550335) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No.550880). The clone HM β1-1 is not recommended for formalin-fixed paraffin embedded sections. Clone HMβ1 also works on rat acetone fixed frozen sections (tested during development).