BD, 550566, BD Pharmingen™ Purified Mouse Anti-Rat CD45

Alternative Name: Ptprc; Lca; Leucocyte common antigen; RT7; T200
Reactivity: Rat (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: Leukocyte Common Antigen-enriched Glycoprotein Fraction from Wistar Rat Thymocytes
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 250 µg/ml
RRID: AB_2174555
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The OX-1 antibody specific for rat CD45 is recommended to test for immunohistochemical staining of Zinc-fixed paraffin sections. Isotype control recommended for use with this antibody is purified mouse IgG1 (Cat. No. 550878). For optimal indirect immunohistochemical staining, the OX-1 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal biotin conjugated anti-mouse Igs (multiple adsorbed) (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat No 550946) together with the DAB detection system (Cat No 550880) .
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. An isotype control should be used at the same concentration as the antibody of interest. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.