BD, 550644, BD Pharmingen™ Purified Mouse Anti-Rat IL-6

Reactivity: Rat (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Recombinant Rat IL-6
Application: ELISA Capture (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_393802
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Capture: The purified C3-4 antibody (Cat. No. 550644) is useful as a capture antibody for a sandwich ELISA for specifically measuring rat IL-6 protein levels. Purified C3-4 antibody can be paired with the biotinylated G307-2 antibody (Cat. No. 550642) as the detection antibody and with recombinant rat IL-6 protein (Cat. No. 557008) as the standard. The purified C3-4 capture antibody should be titrated between 2 - 8 µg/ml to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of rat IL-6 ranging from ~10,000 to 78 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols sections or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site,w ww.bdbiosciences.com. Note 1 : This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring rat IL-6 in serum or plasma our rat IL-6 BD OptEIA™ ELISA Set (Cat. No. 550319) is especially formulated and recommended. Note 2: This ELISA pair shows no cross-reactivity with rat IL-2, IL-4, IL-10, IFNγ, GM-CSF, TNF, mouse or human IL-6.