BD, 550733, BD Pharmingen™ Purified Rabbit Anti-Human CD88

Alternative Name: C5a Receptor
Reactivity: Human (QC Testing)
Isotype: Rabbit IgG
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
RRID: AB_393854
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The purified C85-2506 clone can be used for immunohistochemistry to detect expression of the human C5aR. The antibody has been tested on frozen and fixation of paraffin sections of human tonsil and paraffin sections of human spleen, liver, colon and lung. The dilution range recommended for this antibody is 1-10 to 1-20. The C85-2506 clone does not cross react with C5aR expressed on mouse, rat, pig or monkey tissue. Using biotinylated donkey anti-rabbit Ig (Jackson ImmunoResearch) as the secondary antibody, followed by a peroxidase-based detection system, the expression of C5aR was observed on dendritic cells, granulocytes and monocytes of human tonsil.