BD, 550767, BD Pharmingen™ PE Goat Anti-Rat Ig

Reactivity: Rat (QC Testing)
Isotype: Goat Ig
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_393876
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
Recommended Assay Procedures: Recommended Assay Procedures This antibody conjugate has been tested by immunofluorescent staining (≤ 1.0 µg/million cells) with flow cytometric analysis and as a second-step reagent on mouse splenocytes. This antibody stains rat peripheral B cells, and it has little reactivity with rat non-B splenocytes or mouse splenocytes. As a second step, it is reactive with rat IgG and IgM monoclonal antibodies; a weaker signal is detected when the primary antibody has a rat IgG2b isotype. It has weak cross-reactivity detectable by flow cytometry with some, but not all, hamster immunoglobulins. Consequently, it may be useful as a primary reagent in immunofluorescent staining of rat antibody-producing cells or as a secondary reagent for staining of mouse leukocytes after reaction with rat Ig primary antibodies. However, we have observed that the reactivity of polyclonal second-step antibodies to mouse or rat IgM may be reduced after adsorption against Ig of rat or mouse, respectively. Because this anti-rat Ig antibody was adsorbed with mouse Ig, it may be weakly reactive with some rat IgM primary antibodies. In those cases, we recommend PE Mouse Anti-Rat IgM (Cat. No. 553888) or PE Mouse Anti-Rat Ig, κ Light Chain (Cat. No. 553873). BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.