BD, 550826, BD Pharmingen™ APC Goat Anti-Mouse Ig (Multiple Adsorption)

Reactivity: Mouse (QC Testing)
Isotype: Goat Ig
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_398465
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody solution was passed through solid-phase immunoadsorbent gels to minimize cross-reactivity with rat, human, bovine and horse serum proteins.
Recommended Assay Procedures: Recommended Assay Procedures As a second-step reagent for staining rat spleen and bone marrow leukocytes, it is reactive with mouse primary antibodies having the IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA isotypes. Minimal background staining of rat leukocytes occurs in the absence of primary antibody. In addition, it stains mouse peripheral B lymphocytes with little non-specific staining of other splenic leukocytes. Therefore, it is useful as a primary reagent in immunofluorescent staining of mouse B cells and antibody-producing cells and as a secondary reagent for staining rat leukocytes with mouse Ig primary antibodies. However, we have observed that the reactivity of polyclonal second-step antibodies to mouse or rat IgM may be reduced after adsorption against Ig of rat or mouse, respectively. Because this anti-mouse Ig antibody was adsorbed with rat Ig, it may be weakly reactive with some mouse IgM primary antibodies. In those cases, we recommend biotinylated anti-mouse IgM mAb R6-60.2 (Cat. No. 553406) or anti-mouse Ig κ light chain mAb 187.1 (Cat. No. 559750), followed by Streptavidin-APC (Cat. No. 554067).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.