BD, 550947, BD OptEIA™ Human C4a ELISA Kit

Reactivity: Human (QC Testing)
Application: ELISA (Routinely Tested)
Regulatory Status: RUO
Description: Description Activation of the classical or lectin, but not the alternate, complement pathways results in the production of the C4a anaphylatoxin. C4a has been shown to be a weak multi-functional proinflammatory mediator by increasing vascular permeability, and to be spasmogenic. In blood, plasma or serum, once formed, the nascent C4a anaphylatoxin is rapidly cleaved to the C4a-desArg form by the endogenous serum carboxypeptidase N enzyme. Thus, the quantitation of C4a-desArg in plasma or experimental samples should yield a reliable measurement of the level of classical complement pathway activation that has occurred in the test samples under investigation. C4a production in vivo may also signal activation of the classical or the lectin complement pathways in several autoimmune diseases including rheumatoid arthritis, lupus erythematosus, and acute glomerulonephritis as well as in early stages of organ transplant rejections. The BD OptEIATM Human C4a ELISA Kit is for the in vitro quantitative determination of human C4a and the human C4a-desArg in human EDTA plasma, serum and other biological samples. Components Quantity Antibody Coated Wells 1 plate (12 strips x 8 wells) Detection Antibody 15 mL Standards 3 vials, lyophilized Enzyme Concentrate (250x) 150
Preparation And Storage: Preparation And Storage Store unopened reagents at 2-8°C. do not use reagents after expiration date, or if turbidity is evident. Before use, bring all reagents to room temperature (18-25°C). Immediately after use, return to proper storage conditions. Lyophilized standards are stable until expiration date. After reconstitution, immediately aliquot standard stock in polypropylene vials at 50 µl per vial anf freeze at -80°C for up to 6 months. If necessary, store at 2-8°C for up to 8 hours prior to aliquotting/freezing. Do not leave reconstituted standard at room temperature.
Components : Quantity
Antibody Coated Wells : 1 plate (12 strips x 8 wells)
Detection Antibody : 15 mL
Standards : 3 vials, lyophilized
Enzyme Concentrate (250x) : 150
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