BD, 551309, BD Pharmingen™ Purified Rat Anti-Mouse IFN-γ

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1, κ
Immunogen: Mouse IFN-γ protein
Application: ELISA Capture (Routinely Tested), Immunoprecipitation, Neutralization (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394145
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Capture: The AN-18 antibody has been reported to be useful in a sandwich ELISA that measures mouse IFN-γ protein levels. The purified AN-18 antibody (Cat. No. 551309) can be used as a capture antibody when paired with biotinylated R4-6A2 rat anti-mouse IFN-γ antibody (Cat. No. 551506) as the detecting antibody and with recombinant mouse IFN-γ (Cat. No. 554587) as the standard. The purified AN-18 antibody should be titrated 1-4 µg/ml to determine its optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of mouse IFN-γ ranging from 500 to 5 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our website, www.bdbiosciences.com. Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring mouse IFN-γ in serum or plasma our mouse IFN-γ OptEIA™ Set (Cat. No. 551866) or OptEIA Kit (Cat. No. 550582) are specially formulated and recommended. Immunoprecipitation: The AN-18 antibody has been reported to be used for the immunoprecipitation of mouse IFN-γ; however, the antibody is not routinely tested for this application at Pharmingen.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.