BD, 552371, BD Pharmingen™ Purified Rat Anti-Mouse CD172a

Alternative Name: Sirpa; Sirp alpha; Sirpα; Bit; SHPS-1; p84; Ptpns1
Reactivity: Mouse (QC Testing)
Isotype: Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
Immunogen: Membrane fractions from mouse brain
Application: Flow cytometry (Routinely Tested), Blocking, Electron microscopy, Immunofluorescence, Immunoprecipitation (Reported), Western blot (Not Recommended)
Concentration: 0.5 mg/ml
RRID: AB_394371
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Other reported applications include immunofluorescent staining of cell monolayers and frozen tissue sections, immunoaffinity purification of CD172a, blocking of cellular attachment to CD172a, immunoelectron microscopy, immunoprecipitation, and enhancement of phagocytosis by bone marrow-derived macrophages. It is not effective for western blot analysis.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.