BD, 552405, BD Pharmingen™ Purified Rat Anti-Mouse H2-M

Alternative Name: H2-DM
Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1, κ
Immunogen: Recombinant H2-DM protein
Application: Intracellular staining (flow cytometry) (Routinely Tested), Immunoprecipitation (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394380
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures For flow cytometry of leukocytes, it is recommended that Mouse Fc Block™ (purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142) be used. If Mouse Fc Block™ is used, it is important that the second step anti-rat IgG antibody does not cross-react with the 2.4g2 mAb (rat IgG2b, κ);  we have found that FITC-labeled anti-rat IgG1 mAb RG11/39.4 (Cat. No. 553892) is effective.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.