BD, 552407, BD Pharmingen™ Purified Mouse Anti-Mouse Pre-T Cell Receptor α Chain

Alternative Name: Ptcra; pTα; pTa; gp33; pT[a]; pTalpha; pT-alpha; pT-alpha-TCR
Reactivity: Mouse (QC Testing)
Isotype: Mouse C57BL/6 IgG1, κ
Immunogen: Recombinant extracellular (Ig-like) domain of pTα
Application: Flow cytometry (Routinely Tested), Immunoprecipitation (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394381
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures This antibody has been tested by cell-surface immunofluorescent staining ( ≤8µg/million cells) with flow cytometric analysis to assure specificity and reactivity. Since the pTα is expressed at low density on the surface of immature thymocytes, we recommend the use of Mouse Fc Block™ (anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142) and amplification of the staining intensity through the use of a biotinylated second-step antibody, such as anti-mouse IgG1 mAb A85-1 (Cat. No. 553441), followed by a "bright" third-step reagent, such as Streptavidin-PE (Cat. No. 554061).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.