BD, 552819, BD Pharmingen™ Purified Hamster Anti-Mouse IL-12 Receptor β2

Reactivity: Mouse (QC Testing)
Isotype: Armenian Hamster IgG1, κ
Immunogen: Mouse IL-12Rβ2 transfectants
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_394475
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures The purified anti-mouse IL-12 receptor ß2antibody (clone HAM10B9, Cat. No. 552819) can be used for the immunofluorescent staining (≤1 µg antibody/10e6 cells) and flow cytometric analysis of mouse Th1 or NK cells to measure their expressed levels of surface IL-12Rβ2. An appropriate purified immunoglobulin isotype control is clone A19-3 (Cat. No. 553969).
Product Notices: Product Notices Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Since applications vary, each investigator should titrate the reagent to obtain optimal results. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.