BD, 553393, BD Pharmingen™ Biotin Rat Anti-Mouse IgG2b

Alternative Name: Igh-3; gamma2b; Immunoglobulin heavy constant gamma 2B
Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Immunogen: Pooled Mouse Ig
Application: ELISA Detection (Routinely Tested), Flow cytometry, Immunohistochemistry-frozen (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_394831
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures ELISA : For the mouse IgG2b sandwich ELISA, Biotin Rat Anti-Mouse IgG2b is optimal for detection (~2 µg/ml) using Purified Rat Anti-Mouse IgG2b, (Cat. No. 553396) for capture, with Purified Mouse IgG2b, κ Isotype Control, (Cat. No. 557351) as the standard. Immunofluorescent staining : Biotin Rat Anti-Mouse IgG2b may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgG2b, we recommend FITC Rat Anti-Mouse IgG2b, (Cat. No. 553395). Immunohistochemistry : For IHC, we recommend the use of Biotin Rat Anti-Mouse IgG2b, (Cat. No. 550333) in our special formulation for immunohistochemistry.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.