BD, 553656, BD Pharmingen™ Purified Hamster Anti-Mouse CD154

Alternative Name: CD40 Ligand; CD40L; gp39; Ly-62; Tnfsf5; T-BAM; HIGM1; IMD3
Reactivity: Mouse (QC Testing)
Isotype: Armenian Hamster IgG3, κ
Immunogen: Activated mouse Th1 clone D1.6
Application: Flow cytometry (Routinely Tested), Blocking, Immunohistochemistry-frozen (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394976
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures For the detection of mouse CD154 on activated peripheral T cells, it is strongly recommended that T cells be purified before activation. Mouse CD154 is transiently expressed on the surfaces of activated normal T cells and certain T cell clones with a maximal level detected 6-8 hours post-activation. Activation with immobilized anti-CD3e mAb ( e.g ., 145-2C11, Cat. No. 557306/553058, or 500A2, Cat. No. 553238) is sufficient to induce CD154 expression on CD4+ cells. It has been reported that CD8+ cells express CD154 only in response to PMA/Ionomycin treatment. Therefore, for detection of CD154, it is crucial to utilize the proper activation stimuli and to stain cells at the optimal time for CD154 expression. We recommend the use of biotinylated mouse anti-hamster IgG cocktail (Cat. No. 554010) followed by a "bright" second-step reagent, such as Streptavidin-PE (Cat. No. 554061), for optimal detection of CD154.