BD, 553864, BD Pharmingen™ Purified NA/LE Rat Anti-Mouse CD25

Alternative Name: Interleukin-2 receptor alpha chain; IL-2RA; IL-2Rα; Il2ra; IL-2R p55
Reactivity: Mouse (QC Testing)
Isotype: Rat OFA, also known as Outbred OFA IgG1, λ
Immunogen: IL-2-dependent cytolytic mouse T-cell clone B6.1
Application: Flow cytometry (Routinely Tested), Blocking (Reported)
Concentration: 1.0 mg/ml
RRID: AB_395100
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Flow Cytometry: Investigators may also wish to consider using PE Rat Anti-Mouse CD25 (Cat. No. 553866) or Biotin Rat Anti-Mouse CD25 (Cat. No. 553069/550529) coupled with PE Streptavidin (Cat. No. 554061) for samples expressing low levels of CD25. Immunohistochemistry: Investigators are encouraged to consider using Biotin Rat Anti-Mouse CD25 (Cat. No. 553069/550529) for the immunohistochemistry application.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.