BD, 554073, BD Pharmingen™ Purified Mouse Anti-Human MLH1

Reactivity: Human (QC Testing), Mouse (Tested in Development)
Isotype: Mouse IgG2a, κ
Immunogen: Recombinant Human MLH
Application: Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
Target Molecular Weight: 80-85 kDa
Concentration: 0.5 mg/ml
RRID: AB_395227
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include immunoprecipitation (2 µg/1x106 cells) and western blot analysis (1-3 µg/ml). MCF-7 human breast carcinoma (ATCC HTB-22), 293 adenovirustransformed human kidney (ATCC CRL-1673), and NIH/3T3 mouse fibroblast (ATCC CRL-1658) cells are suggested as positive controls. Clone G168-15 (Cat. No. 13271A) is suggested for immunohistochemical analysis of MLH1; clone G168-15 may also be stronger for western blot analysis than clone G168-728 in some assay systems.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.