BD, 554079, BD Pharmingen™ Purified Mouse Anti-Human p16

Alternative Name: p16-INK4, p16-INK4a, ARF, MTS1, CDKN2, CDK4l
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Human p16 Recombinant Protein
Application: Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
Target Molecular Weight: 16 kDa
Concentration: 0.5 mg/ml
RRID: AB_395229
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Western blot: For protocols on Western blotting, please refer to "Cell Biology (WB, IP, IHC, IF)" at our website: http://www.bdbiosciences.com/us/s/resources Endogenous p16 has been detected in several cell lines including Saos-2 human osteosarcoma (ATCC HTB-85), WI-38 human lung fibroblast (ATCC CCL-75), HeLa cervical carcinoma (ATCC CCL-2) and 293 adenovirus immortalized human kidney (ATCC CRL-1573) cells. U-2 OS human osteosarcoma (ATCC HTB-96) and MCF7 human breast carcinoma (ATCC HTB-22) have been reported to have undetectable levels of p16 and are suggested as negative controls.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.