BD, 554285, BD Pharmingen™ Purified Mouse Anti-Human JNK1/JNK2

Reactivity: Human (QC Testing)
Isotype: Mouse IgG2a
Immunogen: Human JNK1 Fusion Protein
Application: Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
Target Molecular Weight: 46 kDa (JNK1), 54 kDa (JNK2)
Concentration: 0.5 mg/ml
RRID: AB_395344
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures G151-666 immunoprecipitates inactive JNK1 and JNK2 kinases, therefore the antibody is not recommended for in vitro kinase assays. G151-666 is most useful for detection of JNK2 or for detection of both JNK1 and JNK2 in the same assay. Clone G151-333 (Cat. No. 554286) appears to be stronger for detection of JNK1 and is generally suggested for most applications involving JNK1. Clone G151-333 can be used to immunoprecipitate an active JNK1 kinase. HeLa cells (ATCC CCL-1) or NIH-3T3 mouse embryonic fibroblasts (ATCC CRL-1658) are suggested as a positive control for western blot analysis.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.