BD, 554410, BD Pharmingen™ Biotin Rat Anti-Mouse IFN-γ

Alternative Name: Ifg; Ifng; IFN-γ; IFN-g; IFN-gamma; Interferon gamma; Type II Interferon
Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1, κ
Immunogen: Mouse IFN-γ Recombinant Protein
Application: ELISA Detection (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_395374
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Detection: The biotinylated XMG1.2 clone is useful as a detection antibody in a sandwich ELISA for measuring mouse IFN-γ protein levels. Biotinylated XMG1.2 antibody can be paired with the purified R4-6A2 antibody (Cat. No. 551216) or purified AN-18 (Cat. No. 551309) as the capture antibody, with recombinant mouse IFN-γ protein (Cat. No. 554587) as the standard. The biotinylated XMG1.2 antibody preparation should be titrated from 0.5-2.0 µg/ml to determine its optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of IFN-γ protein ranging from ~4000 to 30 pg/ml are recommended for inclusion in each ELISA plate. Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems.  For testing IFN-γ in serum or plasma, our BD OptEIA™ Set (Cat. No. 551866) or BD OptEIA™ Kit (Cat. No. 558258) are recommended.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.