BD, 554427, BD Pharmingen™ FITC Rat Anti-Mouse IL-2

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG2b
Immunogen: Mouse IL-2 Recombinant Protein
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_395385
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Recommended Assay Procedure: Immunofluorescent Staining and Flow Cytometric Analysis: The JES6-5H4 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate IL-2 producing cells within mixed cell populations. FITC-, PE-, APC, Alexa Fluor® 488 and Alexa Fluor® 647 conjugated antibodies (Cat. No. 554427; Cat. No. 554428; Cat No. 554429, Cat. No. 557725 and Cat. No. 557736) are especially suitable for these studies. An appropriate rat IgG2b isotype control is Cat. No. 556923. A useful control for demonstrating specificity of staining is pre-blocking with one of the following: 1) recombinant mouse IL-2 (Cat. No. 550069) or 2) unlabeled JES6-5H4 antibody (Cat. No. 554425), prior to staining. For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Use of these products to measure activation antigens expressed on mononuclear cell subsets for the purpose of monitoring immunoregulatory status can fall under one or more claims of the following patents: US Patent Nos. 5,445,939, 5,656,446, 5,843,689; European Patent No. 319,543; Canadian Patent No. 1,296,622; Australian Patent No. 615,880; and Japanese Patent No. 2,769,156. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.