BD, 554508, BD Pharmingen™ Purified NA/LE Mouse Anti-Human TNF

Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Human TNF Recombinant Protein
Application: ELISA (Routinely Tested), Neutralization (Tested During Development)
Concentration: 1.0 mg/ml
RRID: AB_395441
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures ELISA : Purified Mouse Anti-Human TNF antibody (Clone MAb1, Cat. No. 551220) is useful as a capture antibody for a sandwich ELISA for measuring human TNF. The purified MAb1 antibody can be paired with Biotin Mouse Anti-Human TNF mAb ( Clone MAb11, Cat. No. 554511) as the detection antibody, with recombinant human TNF protein (Cat. No. 554618) as the standard. For complex biological samples, such as serum or plasma, investigators are encouraged to use the BD OptEIA™ Human TNF ELISA Set (Cat. No. 555212) or BD OptEIA™ Human TNF ELISA Kit II (Cat. No. 550610).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.