BD, 554547, BD Pharmingen™ Purified NA/LE Mouse Anti-Human IFN-γ

Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Recombinant human IFN-γ
Application: ELISA (Routinely Tested), Neutralization (Tested During Development)
Concentration: 1.0 mg/ml
RRID: AB_395471
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures ELISA : Purified Mouse Anti-Human IFN-γ antibody (Clone NIB42, Cat. No 551221) can be useful as a capture antibody in sandwich ELISA for measuring human IFN-γ protein levels. Purified NIB42 antibody can be paired with Biotin Mouse Anti-Human IFN-γ mAb, (Clone 4S.B3, Cat. No. 554550) as the detection antibody along with recombinant human IFN-γ (Cat. No. 554617) as the standard. For measuring human IFN-γ in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Human IFN-γ ELISA Set (Cat. No. 555142) or  BD OptEIA™ Human IFN-γ ELISA Kit II (Cat. No. 550612).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.